BBa_J70030

BBa_J70030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julie Norville

Date created: 2008-12-07 12:00:00

Date modified: 2015-05-08 01:08:20

A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_J70030_sequence (Version 1)

Description

When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar
still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector

Notes

Can be used to removed protein tails for protein fusions, see BBF RFC 15

XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX XXX ATGATCAC ATGATCTA^XXXXX

XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX ATT ATGATCAC ATGATCTA^XXXXX

Source

this part is designed

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PpiI 1	4,7	feature/misc, sequence_feature
MabI 1	8,14	sequence_feature, feature/dna
PacI 1	14,20	feature/binding, non_covalent_binding_site
PacI3	18,24	feature/binding, non_covalent_binding_site
PacI 2	22,29	feature/binding, non_covalent_binding_site
MabI 2	30,36	feature/dna, sequence_feature
PpiI 2	37,40	sequence_feature, feature/misc
PpiI 2	46,48	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70030/1

Member of these Collections

• //dna/bioscaffold
• iGEM Parts Registry