Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2008-12-07 12:00:00
Date modified: 2015-05-08 01:08:20
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J70030_sequence (Version 1) |
Description
When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scarstill in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
Notes
Can be used to removed protein tails for protein fusions, see BBF RFC 15XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX XXX ATGATCAC ATGATCTA^XXXXX
XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
Source
this part is designed| Sequence Annotation | Location | Component / Role(s) |
| PpiI 1 MabI 1 PacI 1 PacI3 PacI 2 MabI 2 PpiI 2 PpiI 2 | 4,7 8,14 14,20 18,24 22,29 30,36 37,40 46,48 | feature/misc sequence_feature sequence_feature feature/dna feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/dna sequence_feature sequence_feature feature/misc sequence_feature feature/misc |