BBa_J70559

BBa_J70559 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70559

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julie Norville

Date created: 2010-02-17 12:00:00

Date modified: 2015-05-08 01:08:24

N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040

Details

• Types: DnaRegion
• Roles: sequence_feature; Temporary
• Sequences: BBa_J70559_sequence (Version 1)

Description

designed

Notes

will add B0034 and a six his tag in here later with the oligos

Source

after cutting with BsaXI add these inserts

forward1 (J70559-f1): TTCACACATACTAGAGAAAGAGGAGAAATACTAGATGCATCATC
forward2 (J70559-f2): ATCATCATCACAGCAGCGGCcgta
reverse1 (J70559-r1): CTCTAGTATGTGTGAAatt
reverse2 (J70559-r2): gGCCGCTGCTGTGATGATGATGATGATGCATCTAGTATTTCTCCTCTTT

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_J70550	BBa_J70550
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_J70550	209,1290	BBa_J70550
BBa_E0040	1297,2016	GFP

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70559/1

Member of these Collections

• iGEM Parts Registry