BBa_J70593

BBa_J70593 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70593

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Lynch

Date created: 2010-06-17 11:00:00

Date modified: 2015-05-08 01:08:25

RFC12 ATG Head Domain

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_J70593_sequence (Version 1)

Description

A RFC12 compatible part that codes for a single amino acid, methionine. This is a head domain meant to be assembled directly in front of internal domains.

Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal design your own head domain with the first two or three codons included.

Notes

Made with synthetic
5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F
5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Common knowledge.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	start_codon, feature/start

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70593/1

Member of these Collections

• iGEM Parts Registry