BBa_J70604

BBa_J70604 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70604
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2010-06-27 11:00:00
Date modified: 2015-05-08 01:08:25

J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added



    • Details

    Types
    DnaRegion

    Roles
    Measurement

    engineered_region

    Sequences BBa_J70604_sequence (Version 1)

    Description

    This tests adding a protein fusion to the n-terminus of a protein using J70589 as a test case.

    Notes

    Created by cutting part J70589 with BsaXI and adding rbs, 6 his-glycine serine inserts (legacy from prior design)

    forward1 (J70559-f1i): TTCACACATACTAGAGAAAGAGGAGAAATACTAGATGCATCATC

    forward2 (J70559-f2i): ATCATCATCACAGCAGCGGCcgta

    reverse1 (J70559-r1i): CTCTAGTATGTGTGAAatt

    reverse2 (J70559-r2i): gGCCGCTGCTGTGATGATGATGATGATGCATCTAGTATTTCTCCTCTTT

    Source

    Look at part J70575

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J70604/1
     

    Attachments

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