BBa_J70612

BBa_J70612 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70612
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-07-19 11:00:00
Date modified: 2015-05-08 01:08:25

RFC12 Double Terminator



    • Details

    Types
    DnaRegion

    Roles
    Terminator

    terminator

    Sequences BBa_J70612_sequence (Version 1)

    Description

    This is a RFC12 compatible version of BBa_B0015. It is a double terminator with close to 1 forward efficiency and around .62 reverse efficiency. See BBa_B0015 for more information including folding of the terminator and efficiency data.

    Notes

    PCR amplified off of BBa_J04450 with primers:
    GTTTCTT C GAATTC GCGGCCGC T ACTAGT CCAGGCATCAAATAAAACGAAAGGCTC, Term-F
    GTTTCTT C CTGCAG CGGCCGC T GCTAGC TATAAACGCAGAAAGGCCCACCCG, Term-R

    Source

    BBa_B0015

    Sequence Annotation Location Component / Role(s)
    stem_loop
    T7 TE
    PolyA
    		12,58
    96,115
    116,128
    		feature/stem_loop stem_loop
    stem_loop feature/stem_loop
    feature/polya polyA_site

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J70612/1
     

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