Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2010-08-20 11:00:00
Date modified: 2015-05-08 01:08:25
RBS-MBP-GFP
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J70630_sequence (Version 1) |
Description
RBS and maltose binding protein sequence is inserted into part J70423 to create R0010-J70630.This is the sequence that results on the right side of the part.
Notes
the enzyme psr is used to both cut J70423 to insert the rbs mbp part and to cut the amplified rbs mbp partSource
Maltose binding protein was amplified from the NEB vector pMAL-p5x using the primersRBS-MBP-GS-GFP start f
5'- GAA TTC GCG GCC GCT TCT AGA GAC GAA CGC TCT CTA CTA GAT GCC TAG AGT CG
C CCC CTA AGG GCG GAG GTA GGA GAA ACT CAA ATG AAA ATC GAA GAA GGT AAA
CTG GTA ATC TG -3'
RBS-MBP-GS-GFP start r
5'- CTG CAG CGG CCG CTA CTA GTA GTA ATA TAT GTT CGA TAG ATT TTA CGA GAA CC
A GTC TGC GCG TCT TTC AGG -3'
| Sequence Annotation | Location | Component / Role(s) |
| RBS maltose binding protein gly-ser linker E0040-start sequence | 1,35 36,1136 1137,1142 1143,1858 | ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature CDS feature/cds |