BBa_J70634

BBa_J70634 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70634
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2010-11-01 12:00:00
Date modified: 2015-05-08 01:08:25

Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment



    • Details

    Types
    DnaRegion

    Roles
    Other

    sequence_feature

    Sequences BBa_J70634_sequence (Version 1)

    Description

    Approximately 450 bp upstream DNA fragment of M. florum genome from sites 352951-353409 intended as a recombination site for homologous recombination, removing the restriction enzyme and methylase from M. florum.

    Notes

    Avoidance of biobrick restriction sites, possible primer binding sites.

    Source

    PCR from the M. florum genome.
    Primers:
    • ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F
    • ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R


    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J70634/1
     

    Attachments

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