Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2010-12-29 12:00:00
Date modified: 2015-05-08 01:08:25
Test assembly for Grant's parts
| Types | DnaRegion |
| Roles | sequence_feature Temporary |
| Sequences | BBa_J70664_sequence (Version 1) |
Description
Check assembly instructions on design pageNotes
noneSource
First create J70638, then digest M12100 with ES and J70638 with EX.Make sure J70638 and M12100 are on different vectors. (For this method there should be M12100 on all three vectors.)
Ligate cut fragments together.
Red versions of this on the antibiotic resistance of J70638 part should indicate that the assembly occurred properly.
Count ratio and sequence to check.
| Access | Instance | Definition |
| public public | BBa_M12100 BBa_J70638 | BBa_M12100 BBa_J70638 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M12100 BBa_J70638 | 1,896 905,5198 | BBa_M12100 BBa_J70638 |