BBa_J72016

BBa_J72016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll56} in BBb

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J72016_sequence (Version 1)

Description

{rbs_at_1000.6XHis} RBS with an N-terminal 6XHis tag.

Notes

N/A

Source

Overlap Extension PCR (Wobble construction)

Wobble construction of Bmll56 {rbs_at_10000.6XHis>}

Wobble mll56/mll57                (88, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll56         {rbs_at_10000.6XHis>}



mll56    Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)


CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC


mll57    Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)


CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
6XHis tag	45,60	feature/cds, CDS
Rbs_at_1000	6,36	feature/rbs, ribosome_entry_site

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72016/1

Member of these Collections

• iGEM Parts Registry