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BBa_J72017

BBa_J72017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll38} in BBb

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Details

Types	DnaRegion
Roles	CDS Coding
Sequences	BBa_J72017_sequence (Version 1)

Description

{rbs_at_10000.avi} N terminal avi tag: RBS with an avi tag.

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll38 {rbs_at_10000.AP>}



Wobble mll38/mll39                (108, EcoRI/BamHI

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll38         {rbs_at_10000.AP>}



mll38    Forward wobble for {rbs_at_10000.AP>} (Bmll38)

CCATAGAATTCATGAGATCTCACAAACTGATACAAACGGCGGGGAAATCATGGGCCTGAACGATATTTTTG

mll39    Reverse wobble for {rbs_at_10000.AP>} (Bmll38)

CGTTAGGATCCTTCATGCCATTCAATTTTCTGCGCTTCAAAAATATCGTTCAGGCCCATG