BBa_J72018

BBa_J72018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll40} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72018_sequence (Version 1)

Description

{rbs_at_10000.E} N terminal E tag: RBS with an E tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll40 {rbs_at_10000.E>}



Wobble mll40/mll41 (108, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll40 {rbs_at_10000.E>}


mll40 Forward wobble for {rbs_at_10000.E>} (Bmll40)
CCATAGAATTCATGAGATCTCACATAGCGGCGCTAAATAATAGGGAGGCGATTCAATGGGTGCTCCG
mll41 Reverse wobble for {rbs_at_10000.E>} (Bmll40)
CGTTAGGATCCACGTGGTTCCAGTGGATCTGGATATGGAACCGGAGCACCCATTGAATC


Sequence Annotation Location Component / Role(s)
RBS at 10000
E tag
6,41
44,83
feature/rbs ribosome_entry_site
CDS feature/cds
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J72018/1