BBa_J72019

BBa_J72019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll54} in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72019_sequence (Version 1)

Description

{rbs_at_10000.FLAG} N terminal flag tag: RBS with a FLAG tag.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll54 {rbs_at_10000.FLAG>}

Wobble mll54/mll55                (97, EcoRI/BamHI

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll54         {rbs_at_10000.FLAG>}



mll54    Forward wobble for {rbs_at_10000.FLAG>} (Bmll54)

CCATAGAATTCATGAGATCTGGGACAATCCCGGTCTTTAAGAACAGGAATTACATGTCTGAC

mll55    Reverse wobble for {rbs_at_10000.FLAG>} (Bmll54)

CGTTAGGATCCCTTGTCGTCGTCATCCTTGTAATCGTCAGACATGTAATTCCTG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS at 10000	6,39	ribosome_entry_site, feature/rbs
FLAG tag	42,69	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72019/1

Member of these Collections

• iGEM Parts Registry