BBa_J72020

BBa_J72020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll50} in BBb



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J72020_sequence (Version 1)

Description

{rbs_at_10000.HA} N terminal HA tag: RBS with an HA tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll50 {rbs_at_10000.HA>}



Wobble mll50/mll51 (100, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll50 {rbs_at_10000.HA>}


mll50 Forward wobble for {rbs_at_10000.HA>} (Bmll50)
CCATAGAATTCATGAGATCTTAAGCACCCATAGAATACACACCTGGAGGAATAATGTCTTACC
mll51 Reverse wobble for {rbs_at_10000.HA>} (Bmll50)
CGTTAGGATCCCCCAGCGTAGTCTGGGACGTCGTATGGGTAAGACATTATTCCTC


Sequence Annotation Location Component / Role(s)
RBS at 10000
HA tag
6,39
42,75
feature/rbs ribosome_entry_site
feature/cds CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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synbiohub#topLevel
BBa_J72020/1