BBa_J72022

BBa_J72022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll44} in BBb

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J72022_sequence (Version 1)

Description

{rbs_at_10000.myc} N terminal MYC tag: RBS with a myc tag.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap extension (Wobble Construction)

Wobble construction of Bmll44 {rbs_at_10000.myc>}

Wobble mll44/mll45                (102, EcoRI/BamHI

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll44         {rbs_at_10000.myc>}



mll44    Forward wobble for {rbs_at_10000.myc>} (Bmll44)

CCATAGAATTCATGAGATCTACGCAATATCACACGGAACCGGAGGGCTAACTCGCATGTCTGAAC

mll45    Reverse wobble for {rbs_at_10000.myc>} (Bmll44)

CGTTAGGATCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAGACATGCGAGTTAG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS at 10000	6,40	feature/rbs, ribosome_entry_site
MYC tag	44,77	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72022/1

Member of these Collections

• iGEM Parts Registry