Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26
{Bmll34} in BBb
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J72023_sequence (Version 1) |
Description
{rbs_at_10000.S} N terminal S tag with RBS.Notes
N/ASource
Overlap Extension (Wobble Construction)Wobble construction of Bmll34 {rbs_at_10000.S>}
Wobble mll34/mll35 (112, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll34 {rbs_at_10000.S>}
mll34 Forward wobble for {rbs_at_10000.S>} (Bmll34)
CCATAGAATTCATGAGATCTGAAAAGGAACCCACGCCAAGTCCAGGACACGCCATGAAAGAAACCGCTG
mll35 Reverse wobble for {rbs_at_10000.S>} (Bmll34)
CGTTAGGATCCGGAATCCATGTGCTGACGTTCGAATTTAGCAGCAGCGGTTTCTTTCATGG
| Sequence Annotation | Location | Component / Role(s) |
| RBS at 10000 S tag | 6,39 42,87 | ribosome_entry_site feature/rbs feature/cds CDS |