BBa_J72024

BBa_J72024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll48} in BBb



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J72024_sequence (Version 1)

Description

{rbs_at_10000.Strep} N terminal strep tag with RBS.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension (Wobble Construction)

Wobble construction of Bmll48 {rbs_at_10000.Strep>}



Wobble mll48/mll49 (101, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll48 {rbs_at_10000.Strep>}


mll48 Forward wobble for {rbs_at_10000.Strep>} (Bmll48)
CCATAGAATTCATGAGATCTTACAACTACACACTATCTCATACGGAGCCCTAACATGTCTGACAC
mll49 Reverse wobble for {rbs_at_10000.Strep>} (Bmll48)
CGTTAGGATCCTTTTTCGAACTGCGGGTGGCTCCAGGTGTCAGACATGTTAGGGC


Sequence Annotation Location Component / Role(s)
RBS at 10000
Strep Tag
6,40
43,76
ribosome_entry_site feature/rbs
feature/cds CDS
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BBa_J72024/1