BBa_J72025

BBa_J72025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll52} in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72025_sequence (Version 1)

Description

{rbs_at_10000.T7} N terminal T7 tag with RBS.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll52 {rbs_at_10000.T7>}

Wobble mll52/mll53                (98, EcoRI/BamHI

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll52         {rbs_at_10000.T7>}



mll52    Forward wobble for {rbs_at_10000.T7>} (Bmll52)

CCATAGAATTCATGAGATCTGTACCAAATTAAAAAAATCAAAACGGGGTAAGATATGGCTTC

mll53    Reverse wobble for {rbs_at_10000.T7>} (Bmll52)

CGTTAGGATCCACCCATTTGCTGACCGCCAGTCATAGAAGCCATATCTTACCCC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS at 10000	6,40	feature/rbs, ribosome_entry_site
T7 tag	43,73	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72025/1

Member of these Collections

• iGEM Parts Registry