BBa_J72026

BBa_J72026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72026
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll36} in BBb



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_J72026_sequence (Version 1)

Description

{rbs_at_10000.V5} N terminal V5 tag with RBS.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll36 {rbs_at_10000.V5>}



Wobble mll36/mll37 (109, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll36 {rbs_at_10000.V5>}


mll36 Forward wobble for {rbs_at_10000.V5>} (Bmll36)
CCATAGAATTCATGAGATCTGAAGCAATATCTTCACGTATAAGGAGGTATTTCATGGGTAAGCCTATC
mll37 Reverse wobble for {rbs_at_10000.V5>} (Bmll36)
CGTTAGGATCCCGTAGAATCGAGACCCAGCAGAGGGTTAGGGATAGGCTTACCCATGAAATAC


Sequence Annotation Location Component / Role(s)
V5 tag
RBS at 10000
42,84
6,39
feature/cds CDS
feature/rbs ribosome_entry_site
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BBa_J72026/1