BBa_J72027

BBa_J72027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll46} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72027_sequence (Version 1)

Description

{rbs_at_10000.VSV} N terminal VSV tag with RBS.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll46 {rbs_at_10000.VSV>}



Wobble mll46/mll47 (102, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll46 {rbs_at_10000.VSV>}


mll46 Forward wobble for {rbs_at_10000.VSV>} (Bmll46)
CCATAGAATTCATGAGATCTCAATAGAACATTAGATCAGAACGCGAAAGGGAACAATGTACACC
mll47 Reverse wobble for {rbs_at_10000.VSV>} (Bmll46)
CGTTAGGATCCCTTTCCCAGCCTGTTCATCTCTATATCGGTGTACATTGTTCCCTTTC


Sequence Annotation Location Component / Role(s)
RBS at 10000
VSV Tag
6,41
44,77
feature/rbs ribosome_entry_site
feature/cds CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
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BBa_J72027/1