BBa_J72028

BBa_J72028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll76} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72028_sequence (Version 1)

Description

{6XHis!} C terminal 6XHis tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (wobble construction)

Wobble construction of Bmll76 {<6XHis!}



Wobble mll76/mll77 (52, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll76 {<6XHis!}


mll76 Forward wobble for {<6XHis!} (Bmll76)
CCATAGAATTCATGAGATCTCACCAtCAtCACCAtC
mll77 Reverse wobble for {<6XHis!} (Bmll76)
CGTTAGGATCCttaGTGaTGGTGaTGaTGGTGAGATCT


Sequence Annotation Location Component / Role(s)
6XHis tag
6,24
CDS feature/cds
igem#sampleStatus
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igem#status
Planning
 
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BBa_J72028/1