BBa_J72030

BBa_J72030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll62} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72030_sequence (Version 1)

Description

{E!} C terminal E tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll62 {

Wobble mll62/mll63 (73, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll62 {

mll62 Forward wobble for { CCATAGAATTCATGAGATCTGGTGCTCCGGTTCCATATCCAGATCCACTG
mll63 Reverse wobble for { CGTTAGGATCCTTAACGTGGTTCCAGTGGATCTGGATATGG


Sequence Annotation Location Component / Role(s)
E tag
6,45
CDS feature/cds
igem#sampleStatus
It's complicated
igem#status
Planning
 
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BBa_J72030/1