BBa_J72033

BBa_J72033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll70} in BBb



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_J72033_sequence (Version 1)

    Description

    {Strep!} C terminal Strep tag.

    This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Notes

    N/A

    Source

    Overlap Extension PCR (Wobble Construction)

    Wobble construction of Bmll70 {

    
Wobble mll70/mll71                (61, EcoRI/BamHI
    
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
    
Product is pBca9523-Bmll70         {


    
mll70    Forward wobble for {
CCATAGAATTCATGAGATCTacctggagccacccgcagttcgaa
    
mll71    Reverse wobble for {
CGTTAGGATCCttatttttcgaactgcgggtgg


    Sequence Annotation Location Component / Role(s)
    Strep Tag
    		6,33
    		CDS feature/cds

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J72033/1
     

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