BBa_J72034

BBa_J72034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll68} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72034_sequence (Version 1)

Description

{T7!} C terminal T7 tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll68 {

Wobble mll68/mll69 (67, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll68 {

mll68 Forward wobble for { CCATAGAATTCATGAGATCTATGGCTTCTATGACTGGCGGTCAGC
mll69 Reverse wobble for { CGTTAGGATCCttaACCCATTTGCTGACCGCCAGTCATAG


Sequence Annotation Location Component / Role(s)
T7 tag
6,39
CDS feature/cds
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
synbiohub#ownedBy
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BBa_J72034/1