BBa_J72034

BBa_J72034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll68} in BBb



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_J72034_sequence (Version 1)

    Description

    {T7!} C terminal T7 tag.

    This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Notes

    N/A

    Source

    Overlap Extension PCR (Wobble Construction)

    Wobble construction of Bmll68 {

    
Wobble mll68/mll69                (67, EcoRI/BamHI
    
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
    
Product is pBca9523-Bmll68         {


    
mll68    Forward wobble for {
CCATAGAATTCATGAGATCTATGGCTTCTATGACTGGCGGTCAGC
    
mll69    Reverse wobble for {
CGTTAGGATCCttaACCCATTTGCTGACCGCCAGTCATAG


    Sequence Annotation Location Component / Role(s)
    T7 tag
    		6,39
    		CDS feature/cds

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J72034/1
     

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