BBa_J72035

BBa_J72035 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72035
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll60} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72035_sequence (Version 1)

Description

{V5!} C terminal V5 tag.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll60 {

Wobble mll60/mll61 (76, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll60 {

mll60 Forward wobble for { CCATAGAATTCATGAGATCTGGTAAGCCTATCCCTAACCCTCTGCTGGGTC
mll61 Reverse wobble for { CGTTAGGATCCTTACGTAGAATCGAGACCCAGCAGAGGGTTAG


Sequence Annotation Location Component / Role(s)
V5 tag
6,48
CDS feature/cds
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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BBa_J72035/1