Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_J72037_sequence (Version 1)
|
Description
{Med7DNDc102-205!} Protein 7 of the Mediator Complex (MED7) with a stop codon. This version of MED7 was made to be tagged N-terminally.
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Notes
N/A
Source
PCR
Construction of MED7 Basic Part Bmll5
PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-Bmll5
-------------------------------------
MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA
MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG