BBa_J72038

BBa_J72038 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72038

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll7} in BBb

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J72038_sequence (Version 1)

Description

{Med21DC1-132!} Protein 21 of the Mediator Complex (MED21) with a stop codon. This version of MED21 was made to be tagged N-terminally.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR
Construction of MED21 Basic Part Bmll7
PCR MLL79/MLL80 on pBca9523-Bmll6 (405bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-Bmll7
-------------------------------------
MLL79 Forward Biobricking of Bmll7 CCATAGAATTCATGAGATCTatgACCGACCGTCTGACCC
MLL80 Reverse Biobricking of Bmll7 CGTTAGGATCCttaACCGTCAACGAAGTCTTCGATCAG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72038/1

Member of these Collections

• iGEM Parts Registry