BBa_J72039

BBa_J72039 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72039

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll3} in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72039_sequence (Version 1)

Description

{rbs_at_10000.MED7DNDC102-205} Protein 7 of the Mediator Complex (MED7) with an RBS. This version of MED7 was made to be tagged C-terminally.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Polymerase Chain Assembly

Gene Synthesis of Bmll3 {rbs_at_10000.MED7DNDC102-205>}

Pool mll4 through mll15, assemble by PCA

PCR mll4/mll15 on PCA reaction        (384, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll3   {rbs_at_10000.MED7DNDC102-205>}



mll4     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

CCATAGAATTCATGAGATCTCAGCTAAAATCAACGTATTAAG

mll5     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

TTTCGGTAGATTTTTTCATCGCTATGGAGTGCCTTAATACGTTGATTTTAGC

mll6     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

GATGAAAAAATCTACCGAAAACGAATCTACCAACTACCAGTACAAAATCCAG

mll7     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

AGCAGCAGAGATTTCAGCAGTTTACGCAGTTCCTGGATTTTGTACTGGTAGT

mll8     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

CTGCTGAAATCTCTGCTGCTGAACTACCTGGAACTGATCGGTGTTCTGTCTA

mll9     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

TTCAACTTTACGTTCGTACATGTCCGGGTTGATAGACAGAACACCGATCAGT

mll10     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

TGTACGAACGTAAAGTTGAAAACATCCGTACCATCCTGGTTAACATCCACCA

mll11     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

GAGACTGGTGCGGACGGTATTCGTTCAGCAGGTGGTGGATGTTAACCAGGAT

mll12     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

ATACCGTCCGCACCAGTCTCGTGAATCTCTGATCATGCTGCTGGAAGAACAG

mll13     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

ATTTCACGGATTTCACCACGTTTGTATTCCAGCTGTTCTTCCAGCAGCATGA

mll14     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

CGTGGTGAAATCCGTGAAATCGAACAGGTTTGCAAACAGGTTCACGACAAAC

mll15     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)

CGTTAGGATCCTTAAGAGGTCAGTTTGTCGTGAACCTGTTTG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72039/1

Member of these Collections

• iGEM Parts Registry