BBa_J72040

BBa_J72040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll6} in BBb

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J72040_sequence (Version 1)

Description

{rbs_at_10000.Med21DC1-132} Protein 21 of the Mediator Complex (MED21) with an RBS. This version of MED21 was made to be tagged C-terminally.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Polymerase Chain Assembly

Gene Synthesis of Bmll16 {rbs_at_10000.Med21DC1-132>}

Pool mll16 through mll33, assemble by PCA

PCR mll16/mll33 on PCA reaction        (457, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll16   {rbs_at_10000.Med21DC1-132>}



mll16     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

CCATAGAATTCATGAGATCTGAATACCCTACTAAGGACATTC

mll17     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

GTCAGACGGTCGGTCATTTTCTGGCGAATGTCCTTAGTAGGGTAT

mll18     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

AAAATGACCGACCGTCTGACCCAACTCCAAATTTGCCTGGACCAG

mll19     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

GGGTAGCGCAGAACTGTTCGGTCATCTGGTCCAGGCAAATTTGGA

mll20     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

CGAACAGTTCTGCGCTACCCTGAACTACATCGACAAAAACCACGG

mll21     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

TTCGTTAACGGTCAGACGTTCGAAACCGTGGTTTTTGTCGATGTA

mll22     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

AACGTCTGACCGTTAACGAACCGCAGATGTCTGACAAACACGCTA

mll23     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

GAAAACTCTTCCGGCGGAACAACGGTAGCGTGTTTGTCAGACATC

mll24     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

GTTCCGCCGGAAGAGTTTTCTAACACTATTGACGAACTCTCCACC

mll25     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

TCTGACGGGTTTTCAGGATGATGTCGGTGGAGAGTTCGTCAATAG

mll26     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

CATCCTGAAAACCCGTCAGATCAACAAACTGATCGACTCTCTGCC

mll27     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

TTCCTCTGCGGAAACGTCAACGCCTGGCAGAGAGTCGATCAGTTT

mll28     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

TTGACGTTTCCGCAGAGGAACAACTCCGTAAAATCGACATGCTCC

mll29     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

TCTTCAACTTCAACGAGCTTTTTTTGGAGCATGTCGATTTTACGG

mll30     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

AAGCTCGTTGAAGTTGAAGACGAAAAAATCGAAGCTATCAAAAAA

mll31     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

CAACGTGACGCAGCAGTTTTTCTTTTTTTTTGATAGCTTCGATTT

mll32     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

AAAACTGCTGCGTCACGTTGACTCTCTGATCGAAGACTTCGTTGA

mll33     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)

CGTTAGGATCCACCGTCAACGAAGTCTTCGATCAG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72040/1

Member of these Collections

• iGEM Parts Registry