BBa_J72041

BBa_J72041 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72041
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll74} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72041_sequence (Version 1)

Description

{tev} Tev protease cleavage site.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll74 {}



Wobble mll74/mll75 (52, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll74 {}


mll74 Forward wobble for {} (Bmll74)
CCATAGAATTCATGAGATCTGAAAACCTCTATTTTCAAGG
mll75 Reverse wobble for {} (Bmll74)
CGTTAGGATCCACCTTGAAAATAGAGGTTTTCAGATCT


Sequence Annotation Location Component / Role(s)
Tev Cleavage site
6,27
sequence_feature feature/misc
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J72041/1