BBa_J97000

BBa_J97000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J97000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Drew Endy
Date created: 2014-02-04 12:00:00
Date modified: 2015-05-08 01:08:32

IP-Free EiraCFP (Cyan Fluorescent Protein)



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_J97000_sequence (Version 1)

    Description

    This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement.

    Excitation 398nm, Emission 498nm
    Molecular Weight: 26.0kDA, Length: 233 amino acids

    Notes

    DNA 2.0 have created a large set of new colored and fluorescent proteins by back-translating a set of sequences from Genbank, using their proprietary GeneDesigner software. To create these proteins, they simultaneously minimized sequence differences and and derived an E. coli codon bias for high protein expression. Gene fragments were designed to create chimeric genes when pooled. A subset of the resultant chimeras were selected as base genes from which multiple iterations of libraries with targeted substitutions were made. (from DNA 2.0)

    Source

    Non-Aequorea based sequences.

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J97000/1
     

    Attachments

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