BBa_J99008

BBa_J99008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J99008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Bikard

Date created: 2010-10-27 11:00:00

Date modified: 2015-05-08 01:08:32

CI857/pR thermoinducible promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_J99008_sequence (Version 1)

Description

The thermosensitive CI857 mutant of the lambda phage CI repressor controls the expression of pR. In this system, pR is repressed at 30??C and activated at 42??C.

Notes

na

Source

PCR amplified from pHK-11

Rossignol, M., Moulin, L., and Boccard, F. 2002. Phage hk022-based integrative vectors for the insertion of genes in the chromosome of multiply marked escherichia coli strains. FEMS Microbiology Letters 213:45-49.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CI857	39,752	feature/protein, CDS
pR	791,836	promoter, feature/promoter
-35	800,804	operator, feature/operator
-10	823,828	feature/operator, operator
pRM	755,790	feature/promoter, promoter
-10	758,764	feature/operator, operator
-35	780,788	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J99008/1

Member of these Collections

• iGEM Parts Registry