Types | DnaRegion
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Roles | sequence_feature
Other
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Sequences | BBa_K076005_sequence (Version 1)
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Description
This is a protein linker sequence that allows one reporter protein, in our case EYFP, to be linked to another, in our case mKate, under certain conditions and cleaved under certain other conditions.
Notes
This part must be added by means of specially-designed PCR primers.
Source
This part is described in Osborn et al. "A Picornoviral 2A-Like Sequence-Based Tricistronic Vector Allowing for High-Level Therapeutic Gene Expression Coupled to a Dual-Reporter System." Molecular Therapy, Vol. 12, No. 3, Sept. 2005.