BBa_K076005

BBa_K076005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K076005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sequence from publication (annotated)
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:08:32

2A linker



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K076005_sequence (Version 1)

Description

This is a protein linker sequence that allows one reporter protein, in our case EYFP, to be linked to another, in our case mKate, under certain conditions and cleaved under certain other conditions.

Notes

This part must be added by means of specially-designed PCR primers.

Source

This part is described in Osborn et al. "A Picornoviral 2A-Like Sequence-Based Tricistronic Vector Allowing for High-Level Therapeutic Gene Expression Coupled to a Dual-Reporter System." Molecular Therapy, Vol. 12, No. 3, Sept. 2005.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K076005/1