Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sally.Wang (Jingyi Wang)
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:08:33
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase, the second step enzyme in PCBs degradation
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K078007_sequence (Version 1) |
Description
bphB codes 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase, the second step enzyme in PCBs degradation pathway.Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1 Not1, Pst1 and stop condon downstream.
The initial dioxygenase of the dioxin degradation pathway is a three-component enzyme with an atypical electron supply system.dxnA is one part of this muticomponent complex which acts as ring hydroxylase.dxnA harbors two subunits,dxnA1 and dxnA2.
This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector pSB1AC3 provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
Notes
We use it to construct the degradation pathway of PCBs.This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector pSB1AC3 provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
Source
From the plasmids pAIA73 provided by Professor Bernd Holfer from Department of Microbiology, Gesellschaftfiir Biotechnologische Forschung, D-381 24 Braunschweig, Germany.Type: genomic DNA