BBa_K078009

BBa_K078009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K078009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sally.Wang (Jingyi Wang)
Date created: 2008-10-21 11:00:00
Date modified: 2015-05-08 01:08:33

bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K078009_sequence (Version 1)

Description

bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase, which is the forth step enzyme of PCBs degradation pathway.

This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.

Notes

We use this part to construct the degradation pathway of PCBs

This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.

Source

From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3.
Type: genomic DNA

Sequence Annotation Location Component / Role(s)
bphD
1,860
CDS feature/protein
igem#sampleStatus
It's complicated
igem#status
Planning
 
rdf-schema#seeAlso
P47229
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K078009/1