Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sally.Wang (Jingyi Wang)
Date created: 2008-10-21 11:00:00
Date modified: 2015-05-08 01:08:33
bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K078009_sequence (Version 1) |
Description
bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase, which is the forth step enzyme of PCBs degradation pathway.This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.
Notes
We use this part to construct the degradation pathway of PCBsThis is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.
Source
From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3.Type: genomic DNA