BBa_K078101

BBa_K078101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K078101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gang Wu

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:08:33

aromatic compounds regulatory pcbC promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K078101_sequence (Version 1)

Description

This is a natural promoter from Pseudomonasp.DJ-12 activated by a series of aromatic compunds such as biphenyl,
4-chlorobiphenyl, 4-hydroxybiphenyl etl. It initiates transcrption of downstream genes pcbD and pcbC in DJ-12 when exposing to certain concentration of armotic pollutants.
We implement this promoter as a sensor with a reporter gene fusion downstream to detect PCB related pollutant compounds.

Notes

this promoter is directly synthesised in Geneart Corp

Source

This promoter is from genome of Pseudomonasp.DJ-12.

Reference
Construction of transformant reporters carrying fused genes using pcbC promoter of Pseudomona sp DJ-12 for detection of aromatic pollutants. Enviromental Monitoring and Assessment 92: 241-251,2004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PpcbC	1,86	promoter, feature/promoter
RBS	75,80	ribosome_entry_site, feature/rbs
pcbD	87,129	CDS, feature/protein

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K078101/1

Member of these Collections

• iGEM Parts Registry