Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sally.Wang (Jingyi Wang)
Date created: 2008-10-23 11:00:00
Date modified: 2015-05-08 01:08:33
dxnB
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K078102_sequence (Version 1) |
Description
We use this part to construct the degradation pathway of dioxin.This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
Notes
We use this part to construct the degradation pathway of dioxin.This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
Source
an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1.Type: genomic DNA