Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Lei
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:08:34
TCFbs-BMP4
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K080000_sequence (Version 1) |
Description
Induction of BMP4 during early stages of cardiomyocyte differentiation results in inhibitory effects. From previous studies it is not clear whether BMP4 is necessary for cardiomyocyte differentiation. In our circuit, nkx2.5 and gata4 will be induced and initiate the pathways of cardiac differentiation. During normal cardiac muscle differentiation BMP4 is a downstream target of nkx-2.5 and gata4, the expression of which turns on at later stages of cardiac differentiation. To ensure that BMP4 has a delayed expression, we designed BMP4 under the control of minimal promoters of late markers of cardiac muscle differentiation, TCF and alpha cardiac actin.Notes
NoneSource
TCFbs minPro was isolated via PCR from a human lung embryonal carcinoma cell line, NT2 genomic DNA (atcc # CRL-1973).???Gaire, M., et al. Structure and expression of the human gene for the matrix metalloproteinase matrilysn. (1994) JBC 269:3 2032-2040???
From previous studies, we found a minimal promoter sequence where Beta-catenin/tcf4 could bind and control the expression of MMP7 ???Thomas Brabletz, Andreas Jung, Serpil Dag, Falk Hlubek, and Thomas Kirchner. β-Catenin Regulates the Expression of the Matrix Metalloproteinase-7 in Human Colorectal Cancer. 1999. American J. of Path. 155(4):1033-1038???
BMP4
Mus musculus full length cDNA clone from openbiosystems, clone ID# 4219098