BBa_K090501

BBa_K090501 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K090501
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Goodman
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:08:37

Gram-Positive IPTG-Inducible Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K090501_sequence (Version 1)

Description

This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.

Notes

We found the functional annotation in the associated paper:
Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628

The primers we used to extract it (sans biobrick ends) are as follow:
Forward: AGAACAACCTCTGCTAAAATTC
Reverse: AAGCTTAATTGTTATCCGCTC

Source

We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.

Sequence Annotation Location Component / Role(s)
-35
-10
lacOid Operator
43,48
66,71
78,97
feature/promoter promoter
promoter feature/promoter
operator feature/operator
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K090501/1