Types | DnaRegion
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Roles | promoter
Regulatory
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Sequences | BBa_K090501_sequence (Version 1)
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Description
This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
Notes
We found the functional annotation in the associated paper:
Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628
The primers we used to extract it (sans biobrick ends) are as follow:
Forward: AGAACAACCTCTGCTAAAATTC
Reverse: AAGCTTAATTGTTATCCGCTC
Source
We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.