Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K090502_sequence (Version 1)
|
Description
This is a Xylose-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
Notes
We used the following PCR primers to extract the part, sans biobrick ends:
Forward: TTCATGAAAAACTAAAAAAAATATTG
Reverse: TATGTCATATTGTAAGTAAGTTGCAC
Source
We PCRed this part out of the pSG1154 gram-positive shuttle vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE153.