BBa_K090502

BBa_K090502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K090502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Goodman
Date created: 2008-10-27 12:00:00
Date modified: 2015-06-09 09:03:37

Gram-Positive Xylose-Inducible Promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K090502_sequence (Version 1)

Description

This is a Xylose-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.

Notes

We used the following PCR primers to extract the part, sans biobrick ends:
Forward: TTCATGAAAAACTAAAAAAAATATTG
Reverse: TATGTCATATTGTAAGTAAGTTGCAC

Source

We PCRed this part out of the pSG1154 gram-positive shuttle vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE153.

Sequence Annotation Location Component / Role(s)
-35
-10
Xylose Repressor Binding Region
30,35
53,58
68,91
promoter feature/promoter
promoter feature/promoter
operator feature/operator
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K090502/1