BBa_K090503

BBa_K090503 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090503

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-27 12:00:00

Date modified: 2015-06-09 09:03:21

Gram-Positive General Constitutive Promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K090503_sequence (Version 1)

Description

This is a constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.

Notes

We found the functional annotation in the associated paper: Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628

The primers we used to extract it (sans biobrick ends) are as follows:
Forward: AAACGAGGTCATCATTTCCTT
Reverse: CAAATGTAGTCTTTGAAAGTATTACA

Source

We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	43,48	feature/promoter, promoter
-10	66,71	feature/promoter, promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090503/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //regulation
• iGEM Parts Registry