Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Goodman
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:08:37
Gram-Positive Strong Constitutive Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K090504_sequence (Version 1) |
Description
This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
Notes
This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.Source
We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.| Sequence Annotation | Location | Component / Role(s) |
| putative ComK binding region putative SigA -35 putative SigA -10 | 76,88 102,108 125,130 | operator feature/operator feature/promoter promoter promoter feature/promoter |