BBa_K090506

BBa_K090506 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K090506
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Goodman
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:08:37

Bacillus subtilis weak RBS



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_K090506_sequence (Version 1)

Description

This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence.

The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.

Notes

The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.

Source

This part was synthesized directly.

Sequence Annotation Location Component / Role(s)
B. subtilis weak RBS
1,8
feature/rbs ribosome_entry_site
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K090506/1