Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin Feeney
Date created: 2008-06-25 11:00:00
Date modified: 2015-05-08 01:08:37
pLas promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K091117_sequence (Version 1) |
Description
This pLas' promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein.The pLas' promoter serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.
Notes
To minimize leaky transcription, a secondary transcriptional start site was excluded from the design.Source
The sequence of the LasI upstream regulatory region is available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176640| Sequence Annotation | Location | Component / Role(s) |
| -35 Region LasR Binding Site -10 Region Primary Transcriptional Start | 86,91 75,93 113,118 125,125 | sequence_feature feature/misc non_covalent_binding_site feature/binding sequence_feature feature/misc start_codon feature/start |