BBa_K091130

BBa_K091130 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K091130

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pallavi Penumetcha and Kelly Davis

Date created: 2008-07-09 11:00:00

Date modified: 2015-05-08 01:08:38

pLacIQ controlled GFP generator

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K091130_sequence (Version 1)

Description

This device produces GFP under the control of the lacIQ promoter. The lacIQ promoter has a single base substitution in its -35 region and this increases its binding affinity for RNA polymerase.

Notes

See part K091111 for any design considerations.

Source

The lacIQ promoter sequence came from the paper Glascock and Weickert 1998.

Components

Access	Instance	Definition
public	BBa_K091111	BBa_K091111
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K091111	1,56	BBa_K091111
BBa_B0032	65,77	BBa_B0032
BBa_E0040	84,803	GFP
BBa_B0010	812,891	BBa_B0010
BBa_B0012	900,940	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K091130/1

Member of these Collections

• iGEM Parts Registry