Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin Feeney
Date created: 2008-07-17 11:00:00
Date modified: 2015-05-08 01:08:38
pLas' Testing Construct D
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K091152_sequence (Version 1) |
Description
This part contains a constitutively active promoter, rbs, LuxR coding region, and LasR coding region followed by the pLas' promoter and GFP. K091151 generates both LasR and LuxR to test crosstalk and competitive binding with the pLas' promoter in the presence of AI-1 and PAI-1.Notes
pLas' Testing Construct D has the coding region for LuxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct CSource
The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_S01640 BBa_S03954 BBa_S03984 | BBa_J23100 BBa_S01640 lasR BBa_S03984 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_S01640 BBa_S03954 BBa_S03984 | 1,35 44,842 851,1786 1795,2804 | BBa_J23100 BBa_S01640 lasR BBa_S03984 |