pRecA

BBa_K093000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K093000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julian Wiegelmann, Danielle Nash
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:40

pRecA with LexA binding site



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K093000_sequence (Version 1)

Description

This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1

Notes

How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less.

The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.

Source

synthetic. Oligonucleotides were designed to form the double stranded molecule with sticky ends for direct ligation into a biobrick vector.

Sequence Annotation Location Component / Role(s)
-35
-10
LexA binding
11,17
30,39
15,35
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/operator operator
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K093000/1