Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Harvard iGEM 08
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:41
temperature sensitive cI inducible system with GFP reporter and low promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K098987_sequence (Version 1) |
Description
This is a temperature sensitive cI inducible system driven by a low promoter (BBa_J23113) for the repressor and with a GFP reporter. It can be used to regulate the expression of GFP using heat.Notes
This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site.The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]
Source
PGW7 plasmid and Biobricks parts.| Access | Instance | Definition |
| public public | BBa_J23113 BBa_S04138 | BBa_J23113 BBa_S04138 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23113 BBa_S04138 | 1,35 44,1813 | BBa_J23113 BBa_S04138 |