PN

BBa_K100000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K100000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Garrett Tobin

Date created: 2008-08-17 11:00:00

Date modified: 2015-05-08 01:08:41

Natural Xylose Regulated Bi-Directional Operator

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K100000_sequence (Version 1)

Description

This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).

Notes

Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.

Source

Taken from E. Coli genome bases 3728830-3729092

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RNA poly. binding site	21,26	non_covalent_binding_site, feature/binding
RNA poly. binding site	45,50	non_covalent_binding_site, feature/binding
CRP binding site	90,107	sequence_feature, feature/misc
RNA poly. binding site	243,248	feature/binding, non_covalent_binding_site
RNA poly. binding site	264,269	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K100000/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/bidirectional
• //promoter
• //regulation/multiple
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry