Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Garrett Tobin
Date created: 2008-08-17 11:00:00
Date modified: 2015-05-08 01:08:41
Edited Xylose Regulated Bi-Directional Operator 2
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K100002_sequence (Version 1) |
Description
This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence at an intermediate level.Notes
Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).Source
Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.| Sequence Annotation | Location | Component / Role(s) |
| RNA poly. Binding Site RNA poly. Binding Site Scrambled CRP Binding Site RNA poly. Binding Site RNA poly. Binding Site | 45,50 21,26 88,104 243,248 265,270 | non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding sequence_feature feature/misc non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |