"Part X"

BBa_K1001752 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1001752

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vansh Singh

Date created: 2013-05-01 11:00:00

Date modified: 2015-05-08 01:08:41

RFP and RBS behind Lamda Prm Promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K1001752_sequence (Version 1)

Description

This sequence contains a Red Flourescent Protein and Ribosome Binding Site (BBa_S03873) behind the modified Lambda Prm Promoter(BBa_I12006). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed. Use this BioBrick if your team wishes to have the inducible Lamda Prm promoter to control the expression of an RFP.

Notes

We specifically used the modified Lambda Prm Promoter because it was positively regulated.

Source

We use the intermediate part, BBa_S03873, formed from an RFP (BBa_E1010) and an RBS (BBa_B0034).

Components

Access	Instance	Definition
public	BBa_E1010	mRFP1
public	BBa_S03873	BBa_S03873
public	BBa_I12006	Prm +

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E1010	19,699	mRFP1
BBa_S03873	1,724	BBa_S03873
BBa_I12006	733,814	Prm +

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1001752/1

Member of these Collections

• iGEM Parts Registry